Taq dna polymerase is appropriate for use in the amplification of dna from complex genomic, viral, and plasmid templates, rtpcr, sequencing ssdna, and cycle sequencing. This enzyme allows amplification of simple and complex. Gotaq dna polymerase is a proprietary formulation of taq polymerase that gives robust amplification equal to and, in some cases, superior to that of standard taq. Hifi dna polymerase results in superior accuracy for high fidelity pcr applications 100x more accurate than taq polymerase. The relative fidelity of platinum superfi dna polymerase was calculated to be greater than 100x that of taq dna polymerase. The platinum pfx dna polymerase is provided in inactive form, due to specific binding of the platinum antibody. Invitrogen platinum taq hot start dna polymerase provides high specificity and robust yields.
In contrast to the normal pcr, only a primer is used, so only linear growth not exponential is observed. Platinum taq dna polymerase high fidelity is functionally tested in amplification up to 20 kb using 100 ng of human genomic dna as a template. Invitrogen platinum superfi ii dna polymerase is a proofreading dna polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in pcr. Features of platinum ii hotstart pcr master mix 2x include.
No modification to pcr reactions or protocols are necessary. Pcr protocol for taq dna polymerase with standard taq buffer m0273 protocols. Find low per unit cost of taq polymerase, including bulk and prepack at. Mytaq dna polymerase is a high performance polymerase that exhibits more robust amplification than other commonly used polymerases, delivering very high yield over a wide range of pcr templates and making it the ideal choice for most pcr assays. Taq dna polymerase is a thermostable dna polymerase that possesses a 5.
The following guidelines will help ensure the success of pcr using new england biolabs taq dna polymerase for routine pcr. Platinum taq dna polymerase is supplied at the same 5 unit per. This enzyme allows amplification of simple and complex dna templates over a large range of target sizes and provides 6x higher fidelity over taq. A universal primer annealing feature reduces optimization steps and allows for cocycling of all assays. All books are in clear copy here, and all files are secure so dont worry about it. Platinum pfx dna polymerase product information sheet. It is frequently used in the polymerase chain reaction pcr, a method for greatly amplifying the quantity of short segments of dna. Platinum ii taq hotstart dna polymerase thermo fisher. Invitrogen platinum ii hotstart pcr master mix 2x offers platinum ii taq hotstart dna polymerase premixed with platinum ii pcr buffer and dntps for convenient pcr setup. Pcr specificity is improved with the incorporation of platinum automatic hotstart technology. Currently, str typing is the most efficient and the fastest way for. Relative fidelity values of different dna polymerases.
Invitrogen platinum ii taq hotstart dna polymerase is designed to get you to your research destination, faster. Use of different taq dna polymerases for detection of. The enzyme has terminal transferase activity which results in the addition of a single nucleotide adenosine at 3 end of the extension product. The polymerase activity is blocked at ambient temperatures and restored after the initial denaturation step at 94c. Page 3 of 4 pcr protocol the following procedure is suggested as a guideline and starting point when using platinum pfx dna polymerase in any pcr amplification. Prior to use, dilute the low glycerol platinum taq, cg dna polymerase 50 u. Platinum hotstart technology inhibits dna polymerase activity at ambient temperatures, allowing room temperature. Genscript taq dna polymerases are highly thermostable recombinant dna polymerases and ideally suited for routine pcr reactions. Efficiency comparison of seven different taq polymerases used in hemogenetics j. Taq functions at higher temperatures than a classic dna polymerase and, in part, even permits better sequencing results, because the gcrich structures can be broken down better. The polymerase chain reaction pcr method for amplifying selectively discrete segments of dna has found widespread applications in molecular biology, due in part, to the substitution of a thermostable dna polymerase isolated from thermus aquaticus taq 1 for the previously used e. Use platinum taq dna polymerase for the amplification of dna from complex genomic, viral, and plasmid templates, as well as in rtpcr.
Guidelines for pcr optimization with taq dna polymerase. For further processing on its own or in a mixture as part of an ivd method only. Ms2 rna extract was amplified using primers targeting the 160 bp product from figure 6. Taq dna polymerase is recombinant taq dna polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Efficiency comparison of seven different taq polymerases. Invitrogen platinum taq dna polymerase 600 reactions. Platinum superfi dna polymerase is a proofreading dna polymerase that combines fidelity with platinum hotstart technology, and is ideally suited for cloning, mutagenesis, and other applications. L to at least 10 ul in low glycerol platinum taq, cg diluent included in the kit. Superscript iii onestep rtpcr system with platinum taq.
The hot start property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render taq dna polymerase inactive until the initial pcr denaturation step convenience. Taq polymerase has been employed for some time in sequencing. Taq polymerase is one kind of a dna polymerase which can tolerate high temperatures and be available for dna synthesis without degradation. Adding a proofreading enzyme to taq is thought to improve long pcr by excising misincorporated bases that would otherwise stall taq 12. It ensures higher sensitivity, longer pcr products and higher yields compared to conventional taq dna polymerase. Achieve platinum status hold your work to a higher standard platinum taq. Activity is restored after the denaturation step in pcr cycling at 94c, thereby providing an automatic hot start for taq dna polymerase in pcr 1,2,3. Taq dna polymerase is the enzyme most widely used in the polymerase chain reaction pcr. Invitrogen platinum ii taq hotstart dna polymerase is an engineered taq dna polymerase that shows increased resistance to reaction inhibitors originating from sample material or dna purification steps. Platinum taq dna polymerase is a recombinant taq polymerase complexed with a proprietary antibody that blocks the polymerase activity at ambient temperatures. Taq dna polymerase is evaluated in a dna polymerization activity assay that measures the percent of taq dna polymerase inhibition versus an uninhibited control. Platinum ii taq hotstart dna polymerase is designed for universal primer annealing and fast, easy pcr with its unique combination of innovative buffer, highperformance engineered taq dna polymerase, and leading hotstart technology.
Faststart taq dna polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. Taq dna polymerase is designed for the sensitive, reproducible, endpoint detection and analysis of rna molecules by rtpcr. Jumpstart taq dna polymerase with mgcl2 sigmaaldrich. Platinum taq hot start dna polymerase pdf book manual free.
Dreamtaq dna polymerase uses the same reaction setup and cycling conditions as conventional taq dna polymerase. I used the suggested protocol for taq polymerase according to the. Superscriptz iii onestep rtpcr system with platinum. Taq polymerase is suitable for pcr and automated sequencing reactions. Pcr specificity is improved with the incorporation of platinum. After dilution, the low glycerol platinum taq, cg dna polymerase 10 ul can be stored at 4c for use within 12 days. Platinum taq dna polymerase high fidelity is evaluated in a dna polymerization activity assay that measures the percent of taq dna polymerase inhibition versus an uninhibited control. Activity is restored after the denaturation step in pcr cycling at 94c, providing an automatic. Activity is restored after the initial denaturation step in pcr cycling at 94c, providing an automatic hot start and offering increased sensitivity, specificity, and yield. Highest fidelity dna amplification available at 280x higher than taq, q5 offers unparalleled fidelity for your most important samples, but with a protocol and pricepoint that makes it accessible. Platinum taq dna polymerase is recombinant taq dna polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Taq polymerase is a thermostable dna polymerase i named after the thermophilic eubacterial microorganism thermus aquaticus, from which it was originally isolated by chien et al. Upon heat activation for three minutes at 95c, the antibodies denature irreversibly, releasing fully active taq dna polymerase.
Platinum taq dna polymerase is recombinant taq dna polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient. Thermo scientific dreamtaq dna polymerase is an enhanced taq dna polymerase optimized for all standard pcr applications. Taq polymerase, being thermostable, proved ideal for pcr. Scientists realized that thermostable heatstable dna polymerases would be needed for pcr to work efficiently. This enzyme formulation can also be used in larger volume cocktail mixes without difficulty. Platinum ii taq hotstart dna polymerase is designed for universal primer annealing and fast, easy pcr with its unique combina. Platinum taq dna polymerase high fidelity contains recombinant taq dna polymerase, pyrococcus species gbd polymerase, and platinum taq antibody. Add the following components to an autoclaved microcentrifuge tube.
Recommended for targets 5 kb or with 65% gc sequences. Onetaq dna polymerase is an optimized blend of taq and deep vent dna polymerases for use with routine and difficult pcr experiments. It is supplied with 10x standard taq reaction buffer, which is detergentfree and designed to be compatible with existing assay systems. A unique combination of innovative buffer, highperformance taq dna polymerase, and superior hotstart technology enables.
Taq polymerase is a thermostable dna polymerase that is used for pcr in order to amplify dna sequences. The hot start property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render taq dna polymerase inactive until the initial pcr denaturation step. Taq dna polymerase from thermus aquaticus sigmaaldrich. Difference between taq polymerase and dna polymerase. Invitrogen platinum taq dna polymerase high fidelity 100. Platinum green hot start pcr 2x master mix contains. New england biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a stay at home advisory being put in place in massachusetts, usa, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. Download platinum taq hot start dna polymerase book pdf free download link or read online here in pdf. One unit of taqdna polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into dna in 30 minutes at 74c. Even lowabundance dna templates and difficult gcrich.
Product information thermo scientific dreamtaq dna polymerase. Superscript iii onestep rtpcr system with platinum taq dna polymerase life technologies, the qscript onestep sybr green qrtpcr kit quanta, and the transcriptor onestep rtpcr kit roche. Impact of polymerase fidelity on background error rates in. The onetaq reaction buffers and high gc enhancer have been formulated for. Platinum taq hot start dna polymerase pdf book manual. Platinum taq dna polymerase is recombinant taq, complexed with a proprietary antibody that blocks. Read online platinum taq hot start dna polymerase book pdf free download link book now. The five quality features of q5 high fidelity dna polymerase 1. The 5x gotaq green and colorless reaction buffers supplied with gotaq dna polymerase contain mgcl 2 at a concentration of 7. Description the superscriptz iii onestep rtpcr system with platinum. Anerrorrate of2 x 104 mutation per base per duplication would result in an accu. The polymerase chain reaction pcr was developed by chemist kary mullis in the 1980s, as a means to make many copies of dna fragments. Taq platinum polymerase invitrogen, jumpstart taq polymerase. Even lowabundance dna templates and difficult gcrich templates can be successfully amplified for accurate results.
Kapa hifi dna polymerase kapa hifi dna polymerase is a novel, singleenzyme system that exhibits industryleading fidelity and performance when compared with other proofreading polymerases and polymerase blends. Taq dna polymerase is functionally tested for amplification and the absence of doub le and singlestranded endonuclease. Pcr the polymerase chain reaction pcr is a powerful and sensitive technique for dna amplification 1. To get good pcr yield and reduce nonspecific products, how much dna. I have problem with my pcr when i use platinum taq polymerase. Mar 02, 2017 dna polymerases are used in gene cloning, pcr, dna sequencing, snp detection, molecular diagnostics, etc. Q5 q5 hot start highfidelity dna polymerase neb pu pfuultra ii fusion hs dna polymerase agilent pt platinum taq dna polymerase high fidelity invitrogenlife pf phusion hot start flex dna polymerase neb ap accuprime pfx dna polymerase invitrogenlife 670 bp bp q5 highfidelity dna polymerase robust amplification with. Amplification of templates with high gc content, strong secondary structure, low concentrations or which produce products greater than 5 kb may.