Multidrug resistance of gramnegative bacteria constitutes a major obstacle in the antibacterial fight worldwide. The hydromethanolic extract from nauclea diderrichii was the most clastogenicaneugenic fraction with a minimal active concentration mac of 23. Sep 07, 2010 it suggested that flavonoids and saponins were responsible for the antipyretic and antinociceptive effects of nauclea latifolia roots decoction. Antimalarial activities and toxicities of three plants. Abstractthe antimalarial activities of crude extracts and 17 fractions from the partition of 80%methanolic extracts of three plants the stem bark of croton mubango, the stem bark of nauclea pobeguinii and the leaves of pyrenacantha staudtii used as antimalarial remedies in the democratic republic of congo were studied both in vitro against plasmodium falciparum and in mice infected with. Identification of strictosamide, 8 known iridoidindole alkaloids, and a phenol glycoside. Nauclea diderrichii is found in angola, cameroon, central african republic, the republic of the congo, the democratic republic of the congo, ivory coast, gabon, ghana, liberia, mozambique, nigeria, sierra leone, and uganda. Nauclea is a genus of flowering plants in the family rubiaceae. Antibacterial and antibioticresistance modifying activity of. Mesia k1, tona l, mampunza mm, ntamabyaliro n, muanda t, muyembe t, musuamba t, mets t, cimanga k, totte j, pieters l, vlietinck aj.
The species are evergreen trees or shrubs that are native to the paleotropics. Antibacterial and antibioticresistance modifying activity of the. Nauclea pobeguinii is used in traditional medicine as abortive and for the treatment of stomach ache, infectious diseases, jaundice, fever, diarrhea, worm, and malaria. This study explored the cardiovascular effects of an ethanolic extract of a. All structured data from the file and property namespaces is available under the creative commons cc0 license. The leaves of nauclea pobeguinii are used for the treatment of malaria in the dr congo while the stem bark is used for the prevention of threatened abortion in upper nyong valley in cameroon. Hydromethanolic extracts from nauclea latifolia, nauclea diderrichii and nauclea pobeguinii exhibited a significant clastogenicaneugenic activity without s9 mix. Recently, the plant was shown to have cytotoxic effects on various hematological and carcinoma cell lines.
Extracts from nauclea pobeguinii are active in both rodent models and patients. The discovery of new and effective antimicrobials andor resistance modulators is necessary to combat the spread of resistance or to reverse the multidrug resistance. Novel indole derivatives as potential anticancer agents. Medicinal indications of these species often match the speci. Extracts of the plant have exhibited activity against escherichia coli, shigella flexneri, salmonella typhi and staphylococcus aureus which are responsible for gastroenteritis in children. The lowest ic50 value was obtained with the bark extract of n. Nauclea pobeguinii are used in folk medicine for the treatment of different diseases, especially in the case of diabetes associated or not to hypertension. The genus nauclea in africa comprises seven species. Antimalarial efficacy of a quantified extract of nauclea. The aim of this phase iia clinical trial was to assess the efficacy of an 80 % ethanolic quantified extract containing 5. New antidiabetic and freeradical scavenging potential of.
The hymenodicteaenaucleeae clade is a predominantly paleotropical group with 220 species in 28 genera. Validated liquid chromatography mass spectrometry method. It has scented orange flowers in dense globeshaped clusters. This page was last edited on 18 october 2019, at 16. Nov 04, 2014 this page was last edited on 20 december 2019, at 14. Nigerian medicinal plants are known to contain a large variety of chemical structures and some of the plant extracts have been screened for antitrypanosomal activity, in the search for potential new drugs against the illness. The aspidosperma genus apocynaceae represents one of the largest sources of indole alkaloids widely associated with cardiovascular effects. Nauclea pobeguinii e30, the most active and synergistic of all these extracts, and some hydrolates with antimicrobial activity need further exploration for the development of novel. Neolamarckia cadamba, with english common names burflowertree, laran, and leichhardt pine, and called kadam locally, is an evergreen, tropical tree native to south and southeast asia.
Antimalarial activities and toxicities of three plants used. Antidiabetic potential of two medicinal plants used in. Its natural habitat is subtropical or tropical moist lowland forests. Antibacterial and antibioticresistance modifying activity of the extracts and compounds from nauclea pobeguinii against gramnegative multidrug. By definition, antioxidants are complex compounds found in our diet that act as a protective shield for our. Apart from the main constituent, strictosamide, a series of minor constituents was identified, including two new iridoidindole alkaloids, i.
Phytochemical investigation of dichloromethane neutral phase of stem bark of nauclea pobeguinii led to the isolation of a new monoterpene indole. Phylogeny and generic delimitations in the sister tribes. Files are available under licenses specified on their description page. The structures of all isolated compounds were elucidated with various spectroscopic methods such as 1d and 2d nmr, ir, uv and lcmsittof. The control of these infections is increasingly complicated due to development of resistance against available drugs. In this study, the leaves of terminalia ivorensis, carapa procera, fagara macrophylla, anacardium occidentale, ficus spp. Natural products as starting points for future anti. The results of this study demonstrate the cytotoxicity of six cameroonian medicinal plants, canarium schweinfurthii, dischistocalyx grandifolius, tragia benthamii, fagara macrophylla, myrianthus arboreus and nauclea pobeguinii. The aim of this study was to evaluate the antidiabetic potential of. The crude extracts were subjected to qualitative phytochemical screening.
Compounds 5c and 5d exhibit excellent activity with the ic50 value of. Pdf antimalarial activity and toxicity evaluation of a. This study was initiated to screen the antimicrobial activities of methanol. Subsequently they were evaluated for anticancer activity against cervical cancer hela cell line using mtt assay. Pdf traditional uses, phytochemistry and pharmacological.
The generic name is derived from the ancient greek words naus, meaning ship and kleio, meaning to close. This page was last edited on 20 december 2019, at 14. It has many common names, including bur tree, canary wood, leichhardt pine and yellow cheesewood. Ethnobotanical survey on medicinal plants used by guinean. Cytotoxicity of selected cameroonian medicinal plants and. Download fullsize image highlights characterisation of the total stem bark extract of nauclea pobeguinii by uplcms and hplcspenmr. Nauclea orientalis is a species of tree in the rubiaceae family, native to southeast asia, new guinea, and australia. A new method was developed and validated for the quantification of strictosamide in the extract of the stem bark of nauclea pobeguinii.
In vitro antiinflammatory, antioxidant and in vivo antiarthritic properties of stem bark extracts from nauclea pobeguinii rubiaceae in rats. Natural products as starting points for future antimalarial therapies. Antimalarial activity and toxicity evaluation of a quantified nauclea pobeguinii extract. Diabetes mellitus is a metabolic disorder which is rising globally in rich and developing countries. African trypanosomiasis is a vectorborne parasitic disease causing serious risks to the lives of about 60 million people and 48 million cattle globally. The emergence of multidrug resistant, extensively drug resistant and lengthy therapy reduces the patient compliance and therefore comprises control strategies. In gabon, the species nauclea diderrichii and sarcocephalus pobeguinii syn.
Antimalarial activity and toxicity evaluation of a. Petit rubiaceae, a plant used in traditional medicine in dr congo against malaria. Article views are the countercompliant sum of full text article downloads since november 2008 both pdf and html across all institutions and individuals. Nauclea pobeguinii was investigated, as it was used in folk medicine against different diseases, for example, diabetes mellitus, hypertension, and urogenital infections. Antimalarial activity and toxicity evaluation of a quantified. These metrics are regularly updated to reflect usage leading up to the last few days. Antimicrobial activities of some medicinal plants against. Nauclea diderrichii is found in angola, cameroon, central african republic, the republic of the congo, the democratic republic of the congo, ivory coast, gabon, ghana.
Despite a noticeable progress in the treatment of diabetes mellitus by synthetic drugs, the search for new natural antidiabetic agents is going on. Cancer remains a major health hurdle worldwide and has moved from the third leading cause of death in the year 1990 to second place after cardiovascular disease since 20. The present study provides information on the possible use of nauclea pobeguinii and compound 4 in the control of gramnegative bacterial. Aspidosperma fendleri, a plant found mainly in venezuela, has a single phytochemical report in which is revealed the presence of alkaloids in its seeds.
The terminal vegetative buds are usually strongly flattened. Its antiplasmodial activity has been reported before. In this study, we investigated the antibacterial and antibioticresistance modifying activities against 29 gram. Spotlight on resistant cells and molecular targets. Nauclea latifolia extract in hot water, cold water, petroleum ether and chloroform at concentrations of 200, 150, 100, 50% were tested on some pathogenic bacteria such as staphylococcus aureus, escherichia coli, salmonella typhi and pseudomonas aeruginosa. Clinical trials with cochlospermum planchonii and nauclea pobeguinii showed that these two plant species can be considered as promising candidates for the development of herbal medicines in the treatment of uncomplicated falciparum malaria benoitvical et al. Antimalarial activity and toxicity evaluation of a quantified nauclea pobeguinii extract article in journal of ethnopharmacology 11. The antimalarial efficacy of stem bark extract of nauclea pobeguinii in human adult volunteers with diagnosed uncomplicated falcip arum malaria was also. These results justify the use of these plants in traditional medicine and the practice of supplementing decoctionsconcoctions with conventional antibiotics. Characterization of flowering locus t1 ft1 gene in brachypodium and wheat. Citations are the number of other articles citing this article, calculated by crossref and updated daily. Traditional uses, phytochemistry and pharmacological.
A research paper, showing the presence of a natural painrelieving opoid in the bark and roots of nauclea latifolia. Its bark is deeply furrowed and the heartshaped, deep green leaves are up. Pdf nauclea pobeguinii and uapaca togoensis has the. Antimalarial efficacy of a quantified extract of nauclea pobeguinii stem bark in human adult volunteers with diagnosed uncomplicated falciparum malaria. Jun 01, 2012 the study showed a significant decreased parasitaemia in patients treated with pr 29 ct1 and artesunateamodiaquine with adequate clinical parasitological responses apcr at day 14 of 87. Ethnopharmacological uses of nauclea pobeguinii and nauclea diderrichii are also heavily documented whereas others species were largely left behind. Pdf in vitro antiinflammatory, antioxidant and in vivo.
Antibacterial and antibioticresistance modifying activity. Four new alkaloids, nauclealines a 1 and b 2 and naucleosides a 3 and b 4, together with six known compounds, strictosamide 5, vincosamide 6, pumiloside 7, kelampayoside a, sitosterol, and sitosteryl. Pdf antibacterial and antibioticresistance modifying. Development and validation of an hplcmethod for the. According to the promising results of the phase i and phase iia clinical trials with the herbal medicinal product pr 259 ct1 consisting of an 80 % ethanolic extract of the stem bark of nauclea pobeguinii containing 5. Read development and validation of an hplcmethod for the determination of alkaloids in the stem bark extract of nauclea pobeguinii, talanta on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
It grows to around 35m to 48m tall, and 1m to 2m in. This study investigates the potential for vegetative propagation and clonal selection, and raises some fundamental questions about the physiology of apical dominance and of plagiotropism. For example, the bark of the plant nauclea pobeguinii, from the democratic republic of congo, can substantially reduce parasitemia in mice infected with rodent malaria 97 and has shown efficacy in man in phase iib clinical trials 98. In this study, a new apoptotic monoterpenoid indole alkaloid, subditine 1, and four known compounds were isolated from the bark of nauclea subdita. Antipyretic and antinociceptive effects of nauclea latifolia. Cytotoxicity of selected cameroonian medicinal plants and nauclea. Sarcocephalus pobeguinii hua ex pobeg synonym of nauclea pobeguinii hua ex pobeg merr.
Nauclea diderrichii is a species of tree of the genus nauclea in the family rubiaceae. Nauclea pobeguinii was investigated, as it was used in folk medicine. Merill rubiaceae, an indigenous hardwood of west africa, is increasingly being grown commercially. Text is available under the creative commons attributionsharealike license. New indole alkaloids from the bark of nauclea orientalis. But it is noted that active components of nauclea latifolia remain to be isolate in the further studies. Abstractthe antimalarial activities of crude extracts and 17 fractions from the partition of 80%methanolic extracts of three plants the stem bark of croton mubango, the stem bark of nauclea pobeguinii and the leaves of pyrenacantha staudtii used as antimalarial remedies in the democratic republic of congo were studied both in vitro against plasmodium falciparum and in mice infected. The present overview deals with the potential of the flora of.
This plant belongs to the rubiaceae family and is widely used in the african traditional medicine against malaria and malarialike symptoms. Cytotoxicity of selected cameroonian medicinal plants and nauclea pobeguinii towards multifactorial drugresistant cancer cells. Phytochemical analysis with free radical scavenging, nitric. Sp are used as traditional medicines in gabon for the treatment of different diseases, especially in the case of diabetes. We also demonstrated the antiproliferative potential of nauclea pobeguinii against drugresistant cancer cell lines. In the african region this rate is the highest, with 20 million diagnosed diabetics. It is known by the common names bilinga, aloma, badi, kusia and opepe. To prepare the anticancer indoles, a series of substituted heteroannulated indole derivatives has been synthesized and characterized by spectral and elemental analysis. Nauclea pobeguinii and uapaca togoensis has the potential to fight against multidrug resistant mdr gram negative bacteria and cancer cells lines. Syzygium jambos displayed antibacterial and antibiotic. The total 80% etoh extract of stem bark of nauclea pobeguinii rubiaceae, was analysed by means of uplcms and hplcspenmr. Chromatographic profiling and identification of two new. Chemotherapy is one of the most widely used treatment modes. A phase ii pilot trial to evaluate cobaty017 safety and.
Pdf cytotoxicity of selected cameroonian medicinal. Complete 1 h and c nmr data of the new compound were reported. However, no antimicrobial activity has been reported. Growing to around 50 ft 15 m, this widely distributed tree has a conical shape and spreading branches. There are other feverreducing folk remedies that could yield a nextgeneration antimalarial. To evaluate the in vitro and in vivo antiplasmodial activity and toxicity of the aqueous and 80% etoh extract of the stem bark of nauclea pobeguinii pob. Hypotensive and bradycardic effects of quinovic acid. Phytochemical analysis with free radical scavenging. The genus name honours french naturalist jeanbaptiste lamarck. Naucline, a new indole alkaloid from the bark of nauclea. Antimicrobial activities of a plethora of medicinal plant. Tuberculosis is an infectious disease that kills approximately three million people annually worldwide. Not open access antimalarial efficacy of a quantified extract of nauclea pobeguinii stem bark in human adult volunteers with diagnosed uncomplicated falciparum malaria.
Urogenital tract infections remain a serious global treat especially in women. Considering the promising results of phase i clinical trials with herbal medicine cobaty017, a phase ii study was conducted with plasmodium falciparum malariainfected patients, for efficacy and safety evaluation of cobaty017 compared with artemetherlumefantrine used as a positive control. The structures of isolated compounds were elucidated with various spectroscopic methods such as 1d and 2d nmr, ir, uv and lcms. Results obtained from a phase i clinical trial on healthy male. Eastern and western africa medicinal plants for cancer therapy. Subditine, a new monoterpenoid indole alkaloid from bark. A new indole alkaloid, naucline 1 together with four known alkaloids, angustine 2, angustidine 3, nauclefine 4 and naucletine 5, were isolated from the bark of nauclea officinalis. The present study was designed to evaluate the antibacterial activities of methanol extracts of bark and leaves of syzygium jambos, as well as their synergistic effects with selected antibiotics against drugresistant grampositive and gramnegative bacteria. In addition to that of alkaloid 1, the complete cnmr data of. Rooting ability was high, with up to 100% rooting in 24. Read validated liquid chromatography mass spectrometry method for quantitative determination of strictosamide in dog plasma and its application to pharmacokinetic study, biomedical chromatography on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Therefore a perpetual search of new antimicrobial molecules is needed to face the challenge of microbial resistance. Comparative antimycobacterial activity on lowenstein.